Table 1.

Frequency of lacZα mutants after in vitro replication of unirradiated or UV-irradiated templates by HeLa, MSU-1.2, or XP variant cell extracts

ExtractTem-plateFluence (J/m2)Amt of dNTP incorporated (pmol)aRF I (% of control)No. of plaques scoredMutant frequency (104)
MutantTotal
HeLa oriL0112 ± 18100662,9101.1
5062 ± 955 ± 1550132,7903.8
10045 ± 840 ± 182338,6505.9
15022 ± 220 ± 7
oriR086 ± 17100764,3201.1
5053 ± 462 ± 82880,3703.5
10034 ± 540 ± 63243,0907.4
15015 ± 118 ± 62325,4609.2
MSU-1.2 oriL0106 ± 8100317,6001.8
5066 ± 762 ± 8715,8904.4
10034 ± 632 ± 62432,5007.4
XP variant oriL042 ± 14100548,6701.0
5013 ± 232 ± 127858,65014.2
1007 ± 117 ± 6
150 <5
oriR046 ± 10100336,4800.8
5012 ± 426 ± 82821,24013.2
  • a dNTP, deoxynucleoside triphosphate. Mean ± standard deviation, three to nine determinations.