Table 4.

Kinds and locations of unequivocally independent point mutations induced in the lacZα gene during replication of UV-irradiated M13mp2-oriL by extracts from HeLa cellsa or MSU-1.2 cellsb

MutantPosition(s)MutationPhotoproduct context (5′→3′)dStrandc
HeLa
 HU63−57C · G→T · AAGCTCACTCLagging
 HU64−57C · G→T · AAGCTCACTCLagging
 HU49−57C · G→T · AAGCTCACTCLagging
 HU59−57C · G→T · AAGCTCACTCLagging
 HU3629C · G→T · ATTTCCTGTGLeading
 HU3488C · G→T · ATTTCCCAGTLeading
 HU3988C · G→T · ATTTCCCAGTLeading
 HU7089C · G→T · ATTTCCCAGTLeading
 HU6790C · G→T · ATTTCCCAGTLeading
 HU5190C · G→T · ATTTCCCAGTLeading
 HU4396C · G→T · AAAACCCTGGLagging
 HU69108C · G→T · ATACCCAACTLagging
 HU33108C · G→T · ATACCCAACTLagging
 HU42108C · G→T · ATACCCAACTLagging
 HU53145C · G→T · ACCAGCTGGCLeading
 HU60149C · G→T · ATACGCCAGCLeading
 HU4730C · G→G · CGTTTCCTGTLeading
 HU3789C · G→G · CTTTCCCAGTLeading
 HU57159C · G→A · TTCTTCGCTALeading
 HU6842T · A→C · GATGGTCATALeading
 HU5591T · A→C · GGTTTTCCCALeading
 HU40106–108ΔCTTACCCAACLagging
MSU-1.2
 NU7−57C · G→T · AAGCTCACTCLagging
 NU3475C · G→T · ATTTACACGLagging
 NU189G · G→T · ATTTCCCAGTLeading
 NU389C · G→T · ATTTCCCAGTLeading
 NU1889C · G→T · ATTTCCCAGTLeading
 NU1190C · G→T · ATTTCCCAGTLeading
 NU35108C · G→T · ATACCCAACTLagging
 NU21108C · G→T · ATACCCAACTLagging
 NU2145C · G→T · ACCAGCTGGCLeading
 NU22−57C · G→A · TAGCTCACTCLagging
 NU3185T · A→C · GCCAGTCACLeading
 NU17131T · A→C · GCGACATCCCLagging
 NU3462–63ΔCCGGCCAGTCLeading
  • a The target genes from 44 mutants derived with HeLa cell extracts were sequenced by standard methods. Of these, eight had no mutation in the target gene and two had large deletions. Twelve background mutations were omitted from the analysis.

  • b The target genes from 23 mutants derived with MSU-1.2 cell extracts were sequenced. Of these, six had no mutation in the target gene, three had background mutations, which were omitted from the analysis, and one had an 11-bp insertion between positions 57 and 58.

  • c Strand, as in Table 3.

  • d The site of the mutation is underlined.