Table 1.

Mapping of in vivo phosphorylation sites of KSR1

HPLC fraction(s)Edman degradationbPhosphoamino acidAmino acid residues spanned by deletion mutant analysisSite identified (sequencea)
123S249–320S297 SKS*HESQLGNR
353S320–424S392 TES*VPDSINNPVDR
376T249–320T274 LKPPRT*PPPPSR
5311T249–320T260 ALHSFITPPTT*PQLR
60–62S424–539S443?
  • a Asterisks indicate sites of phosphorylation.

  • b Cycle which 32P counts per minute were released.