Table 5.

Kinds and locations of unequivocally independent point mutations induced in the lacZα gene during replication of UV-irradiated M13mp2-oriR by XP variant cell extractsa

MutantPosition(s)MutationPhotoproduct context (5′→3′)cStrandb
XR196C · G→A · TAAACCCTGGLeading
XR496C · G→A · TAAACCCTGGLeading
XR3108C · G→A · TTACCCAACTLeading
XR5120C · G→A · TTCGCCTTGCLeading
XR9−57C · G→T · AAGCTCACTCLagging
XR11−57C · G→T · AACGTCACTCLagging
XR688C · G→T · ATTTCCCAGTLagging
XR888C · G→T · ATTTCCCAGTLagging
XR1589C · G→T · ATTTCCCAGTLagging
XR790C · G→T · ATTTCCCAGTLagging
XR1254T · A→A · TGAATTCGTALagging
XR1356T · A→A · TCGAATTCACLeading
XR1070T · A→A · TGTCGTTTTALeading
XU14132–136ΔCCATCCCCCTLeading
  • a The target genes from 28 mutants derived with XP variant cell extracts were sequenced. Of these, six had no mutation in the target gene. Two had background mutations and were omitted from the analysis.

  • b Strand, as in Table 3.

  • c The site of the mutation is underlined.