TABLE 1.

Yeast strains

Strain (type)aGenotypeReference
YPH499 (WT) MAT a ade2-101 his3200 leu21 ura3-52 trp163 lys2-801 38
MR103 (Tom22His) MAT a ade2-101 his3200 leu21 ura3-52 trp163 lys2-801 tom22::TOM22His10-HIS3 24
NZY2 (mtHsp70His) MAT a ade2-101 his3200 leu21 ura3-52 trp163 lys2-801 ssc1::SSC1His6-HIS3MX6 44
Tim21ProtA MAT a ade2-101 his3200 leu21 ura3-52 trp163 lys2-801 tim21::TIM21ProtA-HIS3MX6 4
tim21Δ MAT a ade2-101 his3200 leu21 ura3-52 trp163 lys2-801 tim21::HIS3MX6 4
YPH-BG17-9 (tim17-4) MAT a ade2-101 his3200 leu21 ura3-52 trp163 lys2-801 tim17-4 [YEp351]b 4
YPH-BG17-21 (tim17-5) MAT a ade2-101 his3200 leu21 ura3-52 trp163 lys2-801 tim17-5 [YEp351]b 4
yAC58 (tim17-4 Tim21ProtA) MAT a ade2-101 his3200 leu21 ura3-52 trp163 lys2-801 tim17-4 tim21::TIM21ProtA-HIS3MX6 [YEp351]bThis study
yAC59 (tim17-5 Tim21ProtA) MAT a ade2-101 his3200 leu21 ura3-52 trp163 lys2-801 tim17-5 tim21::TIM21ProtA-HIS3MX6 [YEp351]bThis study
KNT17-8 (Tim17His) MAT a ade2-101 his3200 leu21 ura3-52 trp163 lys2-801 tim17::TIM17His10-HIS3MX6This study
Tim21↑ MAT a ade2-101 his3200 leu21 ura3-52 trp163 lys2-801 [pYeDP10-1-TIM21]This study
MB29 (WT) MAT a ade2 his3 leu2 ura3 trp1 lys2 tim23::LYS2 [YCplac33-TIM23] 2
Tim23His MAT a ade2 his3 leu2 ura3 trp1 lys2 tim23::LYS2 [YCplac111-TIM23N-His6] 2
PRY36 (Tim23ProtA) MAT a ade2 his3 leu2 ura3 trp1 lys2 tim23::LYS2 [pRS414-TIM23ProtA] 14
BY4741 (WT) MAT a his31 leu20 met150 ura30 3
Pam18His MAT a his31 leu20 met150 ura30 pam18::PAM18His10-HIS3MX6This study
  • a WT, wild-type strain.

  • b Possible presence of the empty Yep351 vector cotransformed in a procedure to produce chromosomally integrated tim17 mutants.