TABLE 1.

Yeast strains

StrainGenotypea
LNY150 MAT a /MATα leu2::hisG/leu2::hisG trp1::hisG/trp1::hisG lys2/lys2 his4-N/his4-G ura3/ura3 ho::LYS2/ho::LYS2
MSY311 sum1-i::URA3/sum1-i::URA3 cdc28-as1/cdc28-as1 SMK1-HA::KAN/SMK1-HA::KAN
MSY312 cdc28-as1/cdc28-as1 SMK1-HA::KAN/SMK1-HA::KAN
MSY334 SMK1-HA::KAN/SMK1-HA::KAN
MSY338 sum1-ci::URA3/SUM1 SMK1-HA::KAN/SMK1-HA::KAN
MSY339 sum1-ci::URA3/sum1-ci::URA3 SMK1-HA::KAN/SMK1-HA::KAN
MSY349 sum1-c::URA3/sum1-c::URA3 SMK1-HA::KAN/SMK1-HA::KAN
MSY352 sum1-i::URA3/sum1-i::URA 3SMK1-HA::KAN/SMK1-HA::KAN
MSY351 ZIP1::GFP700/zip1::GFP700 ndt80Δ::LEU2/ndt80Δ::LEU2
MSY362 ZIP1::GFP700/zip1::GFP700
MSY386 zip1::GFP700/zip1::GFP700 sum1-ci::URA3/sum1-ci::URA3
MSY350 ndt80::TRP1-PGAL1-NDT80/ndt80::TRP1-PGAL1-NDT80 ura3::PGPD1-GAL4(848)-ER::URA3
MSY385 ndt80::TRP1-PGAL1-NDT80/ndt80::TRP1-PGAL1-NDT80 ura3::PGPD1-GAL4(848)-ER::URA3 sum1-ci::URA3/sum1-ci::URA3
MSY390 hst1Δ::KAN/hst1Δ::KAN sum1-ci::URA3/sum1-ci::URA3
MSY391 hst1Δ::KAN/hst1Δ::KAN
MSY392 sum1-ci::URA3/sum1-ci::URA3
MSY434 sum1-ci::URA3/sum1-ci::URA3 TRP1-NDT80-trp1::hisG/TRP1-NDT80-trp1::hisG SMK1-HA::KAN/SMK1-HA::KAN
MSY428 sum1-ci::URA3/sum1-ci::URA3 ndt80Δ::LEU2/ndt80Δ::LEU2 TRP1-NDT80-trp1::hisG/TRP1-NDT80-trp1::hisG
MSY426 sum1-ci::URA3/sum1-ci::URA3 ndt80Δ::LEU2/ndt80Δ::LEU2 TRP1-NDT80-trp1::hisG/trp1::hisG
MSY438 sum1-ci::URA3/sum1-ci::URA3 ndt80Δ::LEU2/ndt80Δ::LEU2 TRP1-ndt80-ΔM1-trp1::hisG/TRP1-ndt80-ΔM1-trp1::hisG
MSY444 sum1-ci::URA3/sum1-ci::URA3 TRP1-ndt80-ΔM1-trp1::hisG/TRP1-ndt80-ΔM1-trp1::hisG SMK1-HA::KAN/SMK1-HA::KAN
MSY447 rfm1Δ::KAN/rfm1Δ::KAN
MSY448 rfm1Δ::KAN/rfm1Δ::KAN sum1-ci::URA3/sum1-ci::URA3
MSY458 ndt80Δ::LEU2/ndt80Δ::LEU2
MSY460 ndt80Δ::LEU2/ndt80Δ::LEU2 sum1-c::URA3/sum1-c::URA3
EWY167 sum1-ci::URA3/sum1Δ::KAN
  • a All strains are MATa/MATα diploids in the LNY150 (SK1) genetic background (35). The his4-N or his4-G allelic status of the strains has not been tested unless otherwise indicated.