TABLE 1.

Plasmids used in this study

NameCore plasmidInsert
pGC5pGEX-4T-1DAP1 ORF, bp 4 to 459
pGC4pGEX-4T-1DAP1 ORF, bp 4 to 459 containing the D91G mutation
pJM60/YEp-ERG1YEplac195ERG1 ORF with 290 bp of 5′ genomic sequence
pRC31pCR3.1DAP1 ORF with 200 bp of 5′ genomic sequence
pRC34pCR3.1pRC31 mutated to DAP1D91G
pRC39/DAP1D91G-CENpRS313DAP1 ORF with 200 bp of 5′ sequence and the D91G mutation
pRC41/DAP1-CENpRS313DAP1 ORF with 200 bp of 5′ sequence
pRH4/YEp-ERG11YEplac195ERG11 ORF with 300 bp of 5′ genomic sequence
pRH7pRS316ERG11 ORF with 300 bp of 5′ sequence and a single carboxy-terminal Myc epitope tag