TABLE 1.

Complementation and localization analysis of CENP-H derivatives

CENP-H derivativeCompen- sationaLocalization
InterphaseMetaphase
Full-length CENP-H-GFP+CentromereCentromere
1-225CENP-H-GFP+CentromereCentromere
1-215CENP-H-GFPNucleusCytoplasm, centrosome
1-185CENP-H-GFPCytoplasm, centrosomeCytoplasm, centrosome
1-135CENP-H-GFPNucleusCytoplasm, centrosome
1-85CENP-H-GFPCytoplasmCytoplasm, centrosome
53-235CENP-H-GFP+CentromereCentromere
72-235CENP-H-GFP+CentromereCentromere
83-235CENP-H-GFPNucleusCytoplasm
93-235CENP-H-GFPNucleusCytoplasm
142-235CENP-H-GFPNucleusCytoplasm
72-225CENP-H-GFP+CentromereCentromere
93-185CENP-H-GFPNucleusCytoplasm, centrosome
  • a + or −, normal growth was or was not observed, respectively, after transfection with the CENP-H derivatives into CENP-H-deficient cells.