TABLE 1.

Plasmids used in this study

PlasmidRelevant characteristicsSource or reference
YEpU-FT1ZURA3 2μm FRE(Tec1)-lacZ41
YEpL-FT1ZLEU2 2μm FRE(Tec1)-lacZ41
YEpT-FUS1ZTRP1 2μm FUS1-lacZ5
YEpU-FUS1ZURA3 2μm FUS1-lacZ5
YEpL-FUS1ZLEU2 2μm FUS1-lacZ2
YCpUURA3 CEN2
YCpHHIS3 CEN2
YCpTTRP1 CEN2
YCpU-STE5URA3 CEN STE522
YCpU-STE5VASPURA3 CEN STE5-V763AS861P22
YCpU-STE5F14LURA3 CEN STE5-F14L22
YCpH-STE5HIS3 CEN STE5This study
YCpT3-STE7TRP1 CEN STE72
YCpH-STE11-4HIS3 CEN STE11-T596I2
YCpL-STE11-4LEU2 CEN STE11-T596IThis study
YGU-11ΔNURA3 CEN GAL1-STE11 ΔN9
YCpUGURA3 CEN GAL123
pCJ148/YCpUG-STE5-GSTURA3 CEN GAL1-STE5-GST23
pCJ149/YCpUG-STE5-GST*URA3 CEN GAL1-STE5-GST(C177AC180A)23
YCpTG-STE5TRP1 CEN GAL1-STE5This study