TABLE 5.

Two-hybrid interactions among the S. cerevisiae septins^a

AD fusion	β-Galactosidase activity (Miller units) with DBD fusion^b
AD fusion	CDC3	CDC3-N	CDC3-C	CDC10	CDC11	CDC11-N	CDC11-C	CDC12	CDC12-N	CDC12-C
None^c	14	21	29	38	14	21	26	3	15	24
CDC3	5	8	27	290	5	13	25	2,020	10	1,680
CDC3-N	1	7	29	150	3	12	9	1	4	6
CDC3-C	4	16	25	33	7	40	11	410	1	500
CDC10	99	390	17	31	4	37	14	4	26	11
CDC11	1,460	69	160	610	780	500	7	1,760	630	14
CDC11-N	350	7	24	250	66	1,640	8	1,760	570	19
CDC11-C	12	8	56	42	5	43	12	32	20	3
CDC12	1,630	41	1,600	200	1,150	1,220	1	1,740	45	1,530
CDC12-N	260	270	39	51	920	1,610	27	190	13	19
CDC12-C	40	21	700	21	8	50	13	120	2	830

↵a Two-hybrid assays were performed as described in Materials and Methods.
↵b β-Galactosidase activities were measured on three independent isolates of each strain after growth for 16 h at 30°C in minimal medium containing 1% raffinose and 2% galactose. The average values (in Miller units) are shown. Values that are above 100 Miller units and at least sixfold higher than the corresponding control (pJG4-5 with no insert) are shown in boldface. -N and -C indicate the N- and C-terminal fragments, respectively, of the gene in question (see Materials and Methods for details).
↵c None, pJG4-5 with no insert.