TABLE 5.

Two-hybrid interactions among the S. cerevisiae septinsa

AD fusionβ-Galactosidase activity (Miller units) with DBD fusionb
CDC3CDC3-NCDC3-CCDC10CDC11CDC11-NCDC11-CCDC12CDC12-NCDC12-C
Nonec1421293814212631524
CDC35827290513252,020101,680
CDC3-N17291503129146
CDC3-C4162533740114101500
CDC109939017314371442611
CDC111,4606916061078050071,76063014
CDC11-N350724250661,64081,76057019
CDC11-C12856425431232203
CDC121,630411,6002001,1501,22011,740451,530
CDC12-N26027039519201,610271901319
CDC12-C402170021850131202830
  • a Two-hybrid assays were performed as described in Materials and Methods.

  • b β-Galactosidase activities were measured on three independent isolates of each strain after growth for 16 h at 30°C in minimal medium containing 1% raffinose and 2% galactose. The average values (in Miller units) are shown. Values that are above 100 Miller units and at least sixfold higher than the corresponding control (pJG4-5 with no insert) are shown in boldface. -N and -C indicate the N- and C-terminal fragments, respectively, of the gene in question (see Materials and Methods for details).

  • c None, pJG4-5 with no insert.