TABLE 3.

Suppression of septin mutants by BNI5a

MutantPlasmid typeDegree of suppression on growth medium
YEPDYEPG
cdc3-6Vector
2μ-BNI5
GAL1-BNI5
CDC3+4+4
cdc10-1Vector
2μ-BNI5+1+1
GAL1-BNI5+1
CDC10+4+4
cdc11-6Vector
2μ-BNI5+2+2
GAL1-BNI5+2
CDC11+4+4
cdc12-6Vector
2μ-BNI5+2+2
GAL1-BNI5+2
CDC12+4+4
sep7ΔVector
2μ-BNI5+1+1
GAL1-BNI5+1
SEP7+4+4
  • a The cdc3-6, cdc10-1, and cdc12-6 transformants were tested at 30°C, whereas the cdc11-6 and sep7Δ transformants were tested at 34 and 37°C, respectively. The cdc3-6 transformant was also tested at several other temperatures without detecting any suppression (data not shown). The degree of suppression of the growth defects is scored on an arbitrary scale from +1 to +4. −, no suppression. Strains: cdc3-6, KLY1415; cdc10-1, KLY1798; cdc11-6, KLY1419; cdc12-6, KLY1422; sep7Δ, KLY3937. Plasmids: 2μ-BNI5, pKL1119; GAL1-BNI5, pKL899; CDC3, pKL1061; CDC10, pKL1063; CDC11, pKL1064; CDC12, pKL1072; SEP7, pKL2184. Note that in this strain background, the sep7Δ mutation produces a significant growth defect with a chained-cell morphology at 37°C. The growth media used were YEP-glucose (YEPD) and YEP-galactose (YEPG).