TABLE 2.

Plasmids used in this study

NameDescriptionaSource or reference
pEG202-NLS2μ, HIS3, LexA DBDOrigene Technologies, Rockville, Md.
pGEX-KGGST fusion expression vector27
pJG4-52μ, TRP1, transcriptional AD2
pRS314CEN TRP148
pRS316CEN URA348
YCplac33CEN URA324
YCplac111CEN LEU224
YEp3512μ, LEU230
YCp111-GST/ CDC11CEN LEU2 GAL1-GST-CDC1122
pKL899CEN LEU2 GAL1-BNI5See text
pKL1061YCplac111, CDC3See text
pKL1063YCplac111, CDC10See text
pKL1064YCplac111, CDC11See text
pKL1072YCplac111, CDC12See text
pKL2184YCplac33, SEP7/SHS1See text
pKL1119YEp351, BNI5See text
pKL1120YCplac111, BNI5See text
pKL1754pRS316, HOF1/CYK2-mycSee text
pKL1900pRS314, ADH-YFP-BNI5See text
pKL1901pRS314, YFP-CDC10See text
pKL1993GST-CDC11See text
pKL1995T7-BNI5-His6See text
pGEX-4T/CDC3GST-CDC3See text
pGEX-4T/CDC10GST-CDC10See text
pGEX-4T/CDC11GST-CDC11See text
pGEX-4T/CDC12GST-CDC12See text
  • a 2μ indicates high-copy plasmids, and CEN indicates low-copy plasmids.