Table 1.

Strains and plasmids used

DescriptionProteinExpression vectoraYeast vectorbStrainc
Wild typePab1-1pBAS3059BAS3072YAS2261
ΔRRM1Pab1-100pBAS3221BAS3227YAS2235
ΔRRM2Pab1-101pBAS3222BAS3228YAS2236
ΔRRM3Pab1-102pBAS3223BAS3229YAS2237
ΔRRM4Pab1-103pBAS3224BAS3230YAS2238
ΔCtermPab1-104pBAS3225BAS3231YAS2239
Δ3+4+CtermPab1-105pBAS3226BAS3232NA
Y83V, F170V + Δ3+4+CtermPab1-106pBAS3233NAd NA
Y83V, F170VPab1-16pBAS3066BAS3079YAS2025
  • a Bacterial expression vectors were derived from pET11-d (Invitrogen) and are contained in the indicated DH5α strain.

  • b The yeast vector contains the indicatedpab1 gene in a TRP1 CEN4 vector. These vectors are stored in the indicated DH5α strain.

  • c Yeast strain YAS2031 was transformed with the indicated yeast vector and cured of wild-type PAB1 to yield these strains.

  • d NA, not applicable.