Table 1.

Effect of cycloheximide treatment on MG132-induced thermotolerance inise1 cellsa

Treatment Survival (%)
None (0.1% DMSO)0.08 ± 0.01
MG132 (50 μM)2.5 ± 0.3
Cycloheximide (100 μg/ml)0.1 ± 0.02
MG132 + cycloheximide0.2 ± 0.02
  • a Exponentially growing ise1cells at 30°C were treated with MG132 (50 μM), cycloheximide (100 μg/ml), or MG132 plus cycloheximide for 2 h and then shifted to 52°C for 5 min to measure the cell survival as described in Materials and Methods. Data presented are mean values ± SD from three independent experiments.