Table 3.

Distribution of Tf2-neo and Tf2INFS-neo mobilization events

Event class or subclassaTf2 wt eventsbTf2 IN frameshift eventsc
No. observedMobilizationNo. observedMobilization
% of class% of totalh% of class% of totalh
Transposition7 NDf 33.30ND0.0
HR14ND66.720ND100.0
 LCR857.138.1840.040.0
 Linear cDNA conversion642.828.61050.050.0
 Difficult to interpret0 0 0210.010.0
Multiple events1e ND00ND0
No observable event0ND00ND0
HR targeting of tandem arrays2 or 10d NDND5ND25.0
 LCR(0–8)g NDND480.020.0
 Linear cDNA2ND9.5120.05.0
  • a HR, homologous recombination; LCR, LTR circular recombination.

  • b n = 21.

  • c n = 20.

  • d LCR events with Tf2-neo cDNA (wt) generate tandem arrays; thus, targeting to endogenous tandem arrays does not cause loss of the tandem band, making it impossible to determine by this analysis whether an event occurred at the tandem array or elsewhere. Because of the filled-in BsrGI site in the IN frameshift, however, Tf2INFS-neo targeting of the tandem array can be determined when it causes the loss of theBsrGI site and, hence, the loss of the tandem band.

  • e Event 6.

  • f ND, not determined.

  • g Zero to eight HR events may represent LCR type recombination with the tandem array.

  • h (No. observed/n) × 100.