Table 3.

Quantitation of antibody labelinga

ProteinNo. of gold particles/μm2
CytoplasmicNuclearNuclear pore
ProtA-Mex67p1.51.41.6 (1.0 and 0.6)
ProtA-Mtr2p1.10.60.8 (0.6 and 0.2)
ProtA-Pus1p0.51.90
ProtA-DHFR2.20.30
  • a Quantitation was performed as described in Materials and Methods. For ProtA-Mex67p, 22 cells were analyzed and 354 gold particles were counted. For ProtA-Mtr2p, 22 cells were analyzed and 201 gold particles were counted. For ProtA-Pus1p, 20 cells were analyzed and 202 gold particles were counted. For ProtA-DHFR, 20 cells were analyzed and 168 gold particles were counted. Numbers in parentheses indicate cytoplasmic and nuclear sides of the nuclear pore, respectively.