Table 1.

Analysis of INK4a and ARF expression in cell lines by RT-PCRa

Tissue sourceCell line5-Aza-CdR treatmentExpression of:
ARFINK4a
ColonHCT116++
+++
SW48+
+++
HCT15
+++
LoVo
SW837+
HT-29+
CervixC-33A++
Bladder5637++
T24+
+++
J82++
LymphoidRaji+
+++
CA46+
HL-60+
LiverHep 3B++
PancreasCFPac-1+
+++
  • a Expression of the transcript indicated was scored as present (+) or absent (−) after Southern hybridization with oligonucleotide probes specific for the first exon of each gene. 5-Aza-CdR treatments were at 1.0 μM for 72 h.