Table 3.

Interaction between Snf1 and Mig1 in the two-hybrid systema

LexA hybridGAD hybridβ-Galactosidase activity (U/mg of protein)b
5% GluShift to 0.05% Glu
Snf1GAD53
Snf1K84RGAD43
Snf1Mig1ΔZ1172
Snf1K84RMig1ΔZ172162
Snf1Mig1ΔZS222*S278*S311*191,110
Mig1ΔZS278*S311*S381*11810
LexAMig1ΔZ96
Mig1ΔZS222*S278*S311*65
Mig1ΔZS278*S311*S381*57
  • a Strain CTY10-5d was transformed with plasmids expressing the indicated proteins (see Materials and Methods). Immunoblot analysis showed that LexA-Snf1 and LexA-Snf1K84R are expressed at the same levels. GAD-Mig1ΔZ was used because it does not bind to DNA. Transformants were grown to exponential phase in selective SC medium plus 5% glucose (Glu) and then shifted to SC medium plus 0.05% glucose for 3 h.

  • b β-Galactosidase activity was assayed in protein extracts. Values are averages for 3 to 16 transformants, and standard errors were <19%.