Table 2.

Analysis of mtr2 mutant alleles

GenotypemRNA export defectaLocation of:Complemention byMEX67d Synthetic lethality withnup85ΔeComplemention byNUP85f
Mtr2pbMex67pc
MTR2 NPCNPC
mtr2-9 +CytoplasmCytoplasm++
mtr2-26 +NDCytoplasm++
mtr2-21 +NPCCytoplasm+
  • a Analyzed after 30 min at 37°C. −, no defect; +, defect.

  • b Wild-type or mutant Mtr2p was localized as a fusion protein with GFP after 16 min at 36°C. ND, not determined.

  • c Wild-type Mex67p was localized as a fusion protein with GFP after 16 min at 36°C.

  • d Analyzed at 37°C. −, no complementation; +, complementation.

  • e −, no synthetic lethality; +, synthetic lethality.

  • f Analyzed at 33°C. −, no complementation; +, complementation.