Table 1.

β-Galactosidase activity in testes of transgenic mice

Group and transgenic lineaCopy no.β-Galactosidase activity (U min−1 mg of protein−1)b
A
 β4GalT[−1270/−474]LacZ-140c 49,000 (3)
B
 β4GalT[−1085/−474]LacZ-31053650 (1)
 β4GalT[−1085/−474]LacZ-328345,000 (2)
 β4GalT[−1085/−474]LacZ-330650,000 (2)
 β4GalT[−1085/−474]LacZ-346350,000 (3)
C
 β4GalT[−1270/−619]LacZ-9157650 (1)
 β4GalT[−1270/−619]LacZ-923347,000 (2)
 β4GalT[−1270/−619]LacZ-93053240 (1)
D
 β4GalT[−1085/−628]LacZ-421243,000 (3)
 β4GalT[−1085/−628]LacZ-58253,000 (3)
 β4GalT[−1085/−628]LacZ-6413400 (2)
 β4GalT[−1085/−628]LacZ-6428700 (2)
 β4GalT[−1085/−628]LacZ-6485800 (1)
E
 Nontransgenic0600 (3)
  • a The terminal number denotes the individual founder.

  • b Except for that in testis tissue, the β-galactosidase enzymatic activity in all of the tissues analyzed was comparable to the background level in nontransgenic mice. The number in parentheses is the number of F1 and/or F2 mice analyzed.

  • c The transgene β4GalT[−1270/−474]LacZ was previously referred to as β4GT-LacZ (38).