Table 2.

Plasmids and oligonucleotides used

NameDescription or sequenceReference
Plasmids
 pPL15614-kb fragment containing SSY1 in YCp50This work
 pPL16012.5-kb fragment containing PTR3 in YCp50This work
 pPL1935 kb-EcoRI fragment containing PTR3 in pRS316This work
 pPL288 shr3Δ5::hisG-URA3-kanr-hisGin pBluescript II SK(+) 38
 pPL341 ptr3Δ14::hisG-URA3-kanr-hisGin pUC118 (ΔBamHI-ΔHindIII)This work
 pPL3563.9-kb SpeI/ClaI fragment containing SSY1 in pRS316This work
 pHK002pPL356 ΔXbaI in polylinkerThis work
 pHK003pHK002 without native XbaI inSSY1 This work
 pHK004pHK003 withXbaI site introduced into SSY1 at nucleotides 100–105This work
 pHK005pHK003 with XbaI site introduced into SSY1 at nucleotides 202–207This work
 pHK006pHK003 with XbaI site introduced intoSSY1 at nucleotides 616–621This work
 pHK010 SSY1-HA1 in pRS316 (functional)This work
 pHK013 SSY1-HA1 in pRS202 (functional)This work
 pHK017pPL193 ΔXbaI in polylinkerThis work
 pHK018 PTR3-HA1 in pRS316 (functional)This work
 pHK024pHK017 with XbaI site introduced intoPTR3 This work
 pHK030 ssy1Δ12::hisG-URA3-kanr-hisGin pRS316This work
 pHK033 ssy1-HA2 in pRS316 (nonfunctional)This work
 pHK034 ssy1-HA3 in pRS316 (nonfunctional)This work
Oligonucleotides
 POL93-0255′-CGAAATACACAACTGATAGGCGGATCCAAGCTTGCATCTAAATATATACGTATGTTTAAGG-3′
 POL95-0365′-GGACTGGCTTCCAGATTTGTAGAGGATGC-3′
 POL95-0375′-CTTCGTCCAGCCTTTCTAGAAATGATACAGACGG-3′
 POL95-0385′-GACGCTGAGTTATCTAGAAGTTCAATAC-3′
 POL95-0395′-GATTGAGTTGAATTCTTCTACCACTAC-3′
 POL96-0455′-GACACTATCAGACTCTAGAGTTAAGGAGAAATC-3′
 POL97-0385′-AGAAGATGTTTACCAGCACG-3′
 POL97-0395′-TGTTTTCGTCTTTACCATCT GG-3′
 POL97-0425′-ACGCTTGGTAATAGACGCC-3′
 POL97-0435′-TTCTGTTCTTGTTCTTGTGGTG-3′
 POL97-0485′-TCAGATGATGCTCAGGACG-3′
 POL97-0495′-CTGTCTCTGTTACCAACGG-3′
 POL98-0125′-ATGCTTAAGCATGGTCTGC-3′
 POL98-0135′-TTGTCAACGTGGTACATGG-3′