Table 3.

Amino acid uptake into wild-type, ssy1Δ, andptr3Δ strains

Amino acidUptake
With 4 μM substrate (pmol mg−1min−1)FoldaWith 10 mM substrate (nmol mg−1min−1)Fold
Wild typessy1Δ13ptr3Δ15ssy1Δptr3ΔWild typessy1Δ13ptr3Δ15ssy1Δptr3Δ
Leucine20.2 ± 0.315.5 ± 1.013.9 ± 0.60.80.74.9 ± 0.11.8 ± 0.01.8 ± 0.10.40.4
Phenylalanine17.5 ± 0.45.1 ± 0.45.7 ± 0.00.30.34.6 ± 1.41.7 ± 0.51.9 ± 0.20.40.4
Glutamate17.6 ± 1.16.5 ± 0.24.5 ± 0.30.40.33.6 ± 0.32.2 ± 0.31.9 ± 0.10.60.5
Histidine8.6 ± 0.46.9 ± 0.35.1 ± 0.90.80.69.3 ± 1.15.5 ± 0.05.4 ± 0.20.60.6
Lysine22.8 ± 0.718.9 ± 3.013.2 ± 0.20.80.62.3 ± 0.22.3 ± 0.22.3 ± 0.31.01.0
Arginine245 ± 7.5281 ± 5.5281 ± 7.01.11.11.2 ± 0.21.3 ± 0.21.3 ± 0.21.11.1
  • a Ratio with respect to wild type.