Table 1.

S. cerevisiae strains used in these experiments

StrainGenotypeReference or source
W303a MAT a ade2-1 leu2-3,112 ura3-1 his3-11,15 trp1-1 can1-100 ssd1-1 54
169ts MATα ade2-1 leu2-3,112 ura3-1 his3-11,15 can1-100 ssd1-1 ypt6-1 28
JV7-2aAs W303, rpl30Δ::HIS3(pYE: CEN, URA3 GAL1-RPL30) 58
BL17 MAT a /MATα YPT6/ypt6-1 rpl30Δ::HIS3/RPL30 his3-11,15/his3-11,15 ura3-1/ura3-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/cam1-100 ssd1-1/ssd1-1 (pRS316: CEN,URA3 ACT1-RPL30)This study
BL174 ypt6-1 rpl30Δ::HIS3 (pRS316:CEN, URA3 ACT1-RPL30 [construct A])This study
BL175 ypt6-1 rpl30Δ::HIS3(pRS316: CEN, URA3 ACT1-RPL30 [construct B])This study
BL176 ypt6-1 rpl30Δ::HIS3 (pRS316: CEN,URA3 ACT1-RPL30 [construct C])This study
BL177 ypt6-1 rpl30Δ::HIS3 (pRS316:CEN, URA3 ACT1-RPL30 [construct D])This study
Y260 MAT a ura3-52 leu2 rpb1-1 42
JW1201 MATα ypt6-1 ade2-1 leu2-3,112 ura3-1 his3-11,15 can1-100 ssd1-1 act1Δ::URA3 RPL30-ACT1 This study
YDS2 MAT a leu2 ade2 his3 trp1 ura3(∼W303)D. Shore
YDS714 MATα leu2 ade2 his3 trp1 ura3 sir2Δ::HIS3 D. Shore
JW1210 MATα leu2 ade2 his3 trp1 ura3 sly1-1 sir2Δ::URA3 This study
JW1211 MATα leu2 ade2 his3 trp1 ura3 sly1-1 hst1Δ::URA3 This study
YDS430 MATα leu2 ade2 his3 trp1 ura3 sir3Δ::LEU2 D. Shore
BL180 YPT6 SIR3 This study
BL181 YPT6 sir3Δ::LEU2 This study
BL182 ypt6-1 SIR3 This study
BL183 ypt6-1 sir3Δ::LEU2 This study
YDV122 MATα leu2 ade2 his3 trp1 ura3 sir4Δ::LEU2 hmrΔ::TRP1 D. Shore
BL185 YPT6 SIR4 This study
BL186 YPT6 sir4Δ::LEU2 This study
BL187 ypt6-1 SIR4 This study
BL188 ypt6-1 sir4Δ::LEU2 This study