Table 1.

Identification of the nuclear import signal of hTAPa

hTAP aaGFP fusionGFP–β-Gal fusion
Nuclear accumulationNuclear rim associationLocal-izationNuclear rim association
1–619YesYesNYes
1–60NoNoCNo
61–619YesYesNYes
61–560YesNo
61–507YesNo
61–380YesNoNNo
61–341YesNo
61–273YesNo
61–175YesNo
61–160YesNo
61–140YesNo
61–134YesNo
61–128YesNoNNo
61–120YesNoNNo
61–110NNo
61–100Yes, impairedNoN+CNo
61–80NoNoCNo
67–140YesNo
81–140NoNoCNo
95–140NoNo
1–619 R/A 69–82Yes, impairedYesCYes
61–140 (Δ81–109)Yes, impairedNoCNo
61–140 (Δ81–119)Yes, impairedNoCNo
61–120 R/A 97–105N+CNo
61–140 R/A 69–82NoNoCNo
61–140 R/A 78,81CNo
  • a N, nucleus; C, cytoplasm.