Table 1.

Quantitative dataa

FunctionNameCAImRNAAdjusted mRNAProtein (Glu) (103)Protein (Eth) (103)E/G ratio
Carbohydrate metabolismAdh10.8101971971,2309720.79
Adh20.50400963>20
Cit20.18512.82328812
Eno10.870No Nla 4109742.4
Eno20.8922482486502150.33
Fba10.8681791796406080.95
Hxk1,20.5001310.56246
Icl10.25100671>20
Pdb10.342554133
Pdc10.9032262262802050.73
Pfk10.4655575530.71
Pgi10.68114141601200.75
Pyc10.26010.73734
Tal10.5795511035
Tdh20.9046363430876NR
Tdh30.9244604601,6701,927NR
Tpi10.817No Nla No MetNo Met
Protein synthesisEfb10.7623316.5358362
Eft1,20.801262699540.55
Prt10.30340.7126
Rpa00.7932462462771000.36
Tif1,20.75229292331060.46
Yef30.777363614ND
Heat shockHsc820.58122.9112750.67
Hsp600.38192.335822.3
Hsp820.51721.3521352.6
Hsp1040.30477701612.3
Kar20.439510.1431022.4
Ssa10.70924.33034211.4
Ssa20.8021052133241.5
Ssb1,20.850505027085
Ssc10.52122.668801.2
Sse10.521889648
Sti10.24711.125441.7
Amino acid synthesisAde10.229441427
Ade30.27621.7129
Ade5,70.25721.4144
Arg40.22918.14141
Gdh10.585102714855
Gln10.5241111771041.3
His40.2673315231.5
Ilv50.801661521090.7
Lys90.3324432170.52
Met60.657No Nla 22190800.42
Pro20.248333012
Ser10.25821.2158
Trp50.319552812
MiscellaneousAct10.71054542051640.78
Adk10.531No Nla 4743
Ald60.52033181159
Atp20.42414.1761091.4
Bmh10.32246461911370.72
Bmh20.38411.4134147
Cdc480.30622.43226
Cdc600.29920.8662
Erg200.373559239
Gpp10.603165234158
Gsp10.62133115390.34
Ipp10.620442541470.58
Lcb10.1730.30.81940
Mol10.42300.452016
Pab10.4883341190.47
Psa10.600151514856
Rnr40.497664437
Sam10.494555921
Sam20.4973156320
Sod10.3763636631618
Uba10.212221420
YKL0560.73162622531120.44
YLR1090.5492121930
YMR1160.7774141184400.20
  • a CAI, a measure of codon bias, is taken from the YPD. mRNA, number of mRNA molecules per cell from SAGE data (27); adjusted mRNA, number of mRNA molecules per cell based on both SAGE and chip hybridization (30) (see Materials and Methods); Protein (Glu), number of molecules of protein per cell in YNB-glucose; Protein (Eth), number of molecules of protein per cell in YNB-ethanol; E/G ratio, ratio of protein abundance in ethanol to glucose. The E/G ratio is not given if it was close to 1 or if it was not repeatable (NR) in multiple gels. Some gene products (e.g., Tif1 and Tif2 [Tif1,2]) were difficult to distinguish on either a protein or an mRNA basis; these are pooled. No Nla, there was no suitable NlaIII site in the 3′ region of the gene, and so there are no SAGE mRNA data; No Met, the mature gene product contains no methionines, and so there are no reliable protein data.