Table 1.

A negatively supercoiled DNA template partially compensates for the defects of TFIIF mutants in initiationa

TFIIFLinear DNA (% wt)Supercoiled DNA (% wt)
wt100100
S175A110107
E165A9398
L155A2065
W164A2360
N172A3388
I176A1764
  • ↵a The data from Fig. 3 and 8A (60-min time point) are compared.