Table 3.

Radiation-induced interhomologue recombination inmre11Δ/mre11Δ transformants

PlasmidaIR-induced frequency (n)b
50 Gy150 Gy
AsynchronousG1arrestedG2 arrestedAsynchronousG1arrestedG2 arrested
pScM11-3147.7 ± 2.5 (14)33.5 ± 16.7 (16)7.1 ± 3.5 (16)7.3 ± 1.4 (5)35.0 ± 8.4 (6)4.8 ± 2.2 (6)
pRS31411.1 ± 12.1 (10)49.0 ± 15.6 (8)8.5 ± 15.9 (8)−4.7 ± 1.9 (6)31.5 ± 6.9 (5)−3.2 ± 6.9 (6)
  • a Diploid mre11Δ strain JPY264 was transformed with a native promoter-driven MRE11expression construct (pScM11-314) or an empty vector (pRS314). Details of plasmid construction are given in Materials and Methods.

  • b IR-induced frequencies were calculated by subtracting the spontaneous frequency of canavanine-resistant methionine prototrophs in the unirradiated sample from the frequency in the irradiated sample. Values are given as 10−4recombinants per viable cell ± standard deviations. Numbers in parentheses indicate the number of independent cultures used to determine the given value.