Table 1.

Strains

StrainGenotype (source)
W303-1A MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 LYS2 (R. Rothstein)
W303-1B MATα ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 LYS2 (R. Rothstein)
YCH192W303-1Abar1::URA3
YCH201W303-1ADBF4-proA-HIS3-URA3 bar1::URA3
H14C1A1 MAT a cdc14-1 his7 ura1 (L. Hartwell)
YCH189W303-1A cdc14-1 (H5C1A1 backcrossed two times to W303)
YCH219W303-1A cdc14-1 DBF4-proA-HIS3-URA3 (ProA-tagged DBF4 in YCH189)
K1983 MAT a cdc4-1 ade2 ura3 his3(backcrossed four times to W303) (K. Nasmyth)
YCH215 MAT a cdc4-1 ade2 ura3 his3 DBF4-proA-HIS3-URA3 (ProA-tagged DBF4 in K1983)
K2034-CH-α MATα cdc13-1 ade2 ura3 his3(backcrossed five times to W303) (K. Nasmyth backcrossed three times, and then we backcrossed it an additional three times to W303)
YCH216 MATα cdc13-1 ade2 ura3 his3 DBF4-proA-HIS3-URA3 (ProA-tagged DBF4 in K2034)
K2531 MAT a cdc23-1 ade2 leu2 ura3 his3 TRP1 (backcrossed three times to W303) (K. Nasmyth)
YCH217 MAT a cdc23-1 ade2 leu2 ura3 his3 TRP1 DBF4-proA-HIS3-URA3 (ProA-tagged DBF4 in K2531)
YCH208W303-1A cdc23-1 bar1::URA3(segregant from YCH192 × K2531)
H5C1A1 MAT a cdc5-1 his7 ura1 (B. Gavin and L. Hartwell)
YCH187W303-1A cdc5-1 (H5C1A1 backcrossed five times to W303)
YCH218W303-1A cdc5-1 DBF4-proA-HIS3-URA3(ProA-tagged DBF4 in YCH187)
YCH288W303-1ADBF4-proA-HIS3-URA3 (ProA-tagged DBF4 in W303-1A)
YOC1523W303-1A bar1 PDS1-HA::URA3 mec1-1(O. Cohen-Fix and D. Koshland)
YOC1524W303-1A bar1 PDS1-HA::URA3 rad53-21 (O. Cohen-Fix and D. Koshland)
YCH324W303-1A cdc13-1 DBF4-proA-HIS3-URA3 mec1-1(segregant of YCH216 and YOC1523)
YCH325W303-1A cdc13-1 DBF4-proA-HIS3-URA3 rad53-21 (segregant of YCH216 and YOC1524)
H16C1A1 MAT a cdc16-1 his7 ura1 (B. Gavin and L. Hartwell)
YCH407W303-1B cdc16-1 (H16C1A1 backcrossed five times to W303)
YCH406W303-1A cdc16-1 DBF4-proA-HIS3-URA3 bar1::URA3 (segregant from YCH201 × YCH407)
YCH404W303-1A cdc16-1 bar1::URA3 (segregant from YCH201 × YCH407)