Table 1.

Interaction of Dvl-1, Dvl-3, and Axin in the yeast two-hybrid systema

Proteinβ-Galactosidase activity
Dvl-1-(1-82) (pBTM116HA)Dvl-1 (pBTM116HA)rAxin (pBTM116HA)
Dvl-3 (pGAD)+++
rAxin (pGAD)++
GSK-3β (pGAD)+
β-Catenin (pGAD)+
Ras (pGAD)
  • a L40 cells were cotransformed with pGAD- and pBTM116HA-derived plasmids, as indicated in parentheses. To assay for β-galactosidase activity, cells were streaked on permissive (histidine-containing) medium and incubated for 3 days at 30°C, and then β-galactosidase activity was measured as negative (−) or positive (+). All constructs except Dvl-1-(1-82) are full-length proteins.