Table 1.

Stimulation of transactivation activity of the GAL4–IRF-3 chimeras by Sendai virusa

Cotransfected plasmidRelative CAT activity
ControlSendai virus
pSG42411.3 ± 0.4
IRF-3108 ± 27157 ± 36
IRF-3(407)228 ± 23237 ± 26
IRF-3(394)213 ± 29243 ± 33
IRF-3(151-427)14.2 ± 3.335 ± 7.1
IRF-3(134-427)198 ± 40217 ± 57
IRF-3(99-427)219 ± 56227 ± 46
IRF-3(2A)69 ± 20148 ± 40
IRF-3(3A)190 ± 39206 ± 45
IRF-3(5A)193 ± 42206 ± 46
IRF-3(5D)229 ± 44264 ± 24
IRF-3(J2A)77 ± 1485 ± 24
IRF-3(J2D)69 ± 18172 ± 36
IRF-3(NES)4.9 ± 2.522 ± 8.1
IRF-3(5D-NES)160 ± 17139 ± 33
  • a 293 cells were transfected with 2.5 μg of (Gal4)5-TK-CAT reporter plasmid and 5 μg of the various GAL4–IRF-3 chimeric expression plasmids as indicated. At 24 h posttransfection, cells were infected with Sendai virus for 16 h or left uninfected. CAT activity was analyzed at 48 h posttransfection with 10 μg of total protein extract for 1 h at 37°C. Relative CAT activity was measured as fold activation (relative to the basal level of reporter gene in the presence of pSG424 vector after normalization with cotransfected β-Gal activity); the values represent the average of three experiments ± standard deviation.