Table 3.

Precise religation as a mode of intrachromosomal DSB repair

Cell lineNo. of electroporationsFrequency of HAT-resistant colonies (10−4)aNo. of HAT-resistant colonies analyzedXbaI site loss (%)bPrecise religation (%)c
Sce2-132.2394436
Sce2-212.4176565
Sce2-310.068153327
Sce2-410.03265033
  • a Number of HAT-resistant colonies divided by total number of cells electroporated.

  • b Percentage of analyzed HAT-resistant clones that lost the XbaI site between the I-SceI sites (Fig. 5), based on PCR analysis.

  • c Percentage of analyzed HAT-resistant clones that had undergone precise religation, based on nucleotide sequence determination.