Table 1.

Membranolytic/endosomolytic agents used in this studya

AgentConcn producing >80% specific 51Cr releasebConcn producing 10% specific 51Cr releasecConcn used in apoptosis assays
Perforin1,000–1,500 U/ml300–500 U/ml130–350 U/ml
PLO160 U/ml10–20 U/ml5–20 U/ml
SLO6–12 μg/ml0.5–1.0 μg/ml0.02–0.5 μg/ml
SAT3–6 μg/ml0.1–0.2 μg/ml0.02–5.0 μg/mld
RC25% (vol/vol)1.5–3.0%1.5–25%d
LLO100–200 ng/ml15–30 ng/ml1.2–1.5 ng/ml
  • a The concentrations refer to specific release of 51Cr from Jurkat cells over 4 h at 37°C (see Materials and Methods). The ranges shown were determined from three or four independent experiments performed with different batches of cells and different dilutions of the toxins.

  • b High concentration of toxins used in apoptotic and other assays.

  • c Sublytic concentration of toxins used in apoptotic and other assays.

  • d Not permissive for apoptosis.