Table 2.

Summary of Southern blotting analysis of HAT-resistant segregants from cell lines 26-1 and 26-2

Event type and cell lineNo. of HAT-resistant segregants analyzedNo. of HAT-resistant segregants displaying:
HRNHEJAmplificationa
DSB-induced
 26-1127b 50
 26-2305c 1213
Spontaneous
 26-22020d 00
  • a Thirteen HAT-resistant segregants from cell line 26-2 displayed an amplification of sequences in the vicinity of the I-SceI-induced DSB, in conjunction with NHEJ. These 13 clones are distinct from the 12 segregants from cell line 26-2 listed under NHEJ. See text for details.

  • b Among the recombinants, six were gene conversions of the I-SceI recognition sequence insertion and one was a crossover.

  • c Among the recombinants, all five were gene conversions of the I-SceI recognition sequence insertion.

  • d Among the recombinants, 18 were gene conversions of the I-SceI recognition sequence insertion, 1 was a gene conversion of the XhoI linker insertion, and 1 was a crossover.