Table 1.

Analysis of the interaction between DOK proteins and Abl in the yeast two-hybrid systema

Plasmidβ-Galactosidase activity when cotransformed with plasmid:
AGP3AGP4AGP5ACAGP7AGP8AGP9
pGD-DOKL+++++++++++++++
pGD-DOKLR209A +/−+/−+/−+/−+/−
pGD-DOKLR224A ++++++++++
pGD-DOKLR209, 224A +/−+/−+/−+/−+/−
pGD-p62d o k +++++++++++++++
pGD-p62d o kR207A+/−+/−+/−+/−+/−
  • a Plasmids in the heading contained different DNA fragments in frame with the LexA DNA-binding domain. Plasmids in the left column contained different DNA fragments in frame with the Gal4 activation domain. Yeast strain CTY 10-5d was transformed with various pairs of plasmids, followed by an X-Gal staining assay. The strength of interaction was indicated by the timing and intensity of blue color. +++, dark blue visible in 1 h; ++, dark blue visible in 4 h; +/−, pale blue visible overnight; −, white overnight.