Table 1.

Yeast strains used in this study

StrainaGenotype
SL984-6B MAT a ura3-52 his3-Δ200 leu2-1 met8-1 ilv1-1 trp1-901 cac3-1
L1356 MAT a ura3-52 his3-Δ200 leu2-1 met8-1 ilv1-1 trp1-901 cac3-1 lys2::his3Δ4
L1561L1356hir3-2 (also called htr1)
L1562L1356rad1::LEU2
L1563L1356rad52::URA3
L1635L1356rad6::LEU2
L1675L1356hir3::LEU2
L1680L1356hir1::URA3
L1681L1356hir2::URA3
L1685L1356cac2::TRP1 hir3::LEU2 [pCAC3]
SL1005-54 MATα his3-Δ200 leu2-1 lys2::his3-Δ4 ade1 cac3-1
SL1006-1B MATα his3-Δ200 leu2-1 lys2::his3-Δ4 ura3-52 trp1-901 met8-1 cac3-1 hir3-2
SL1006-1D MATα his3-Δ200 leu2-1 lys2::his3-Δ4 trp1-901 met8-1 cac3-1
SL1006-7B MATα his3-Δ200 leu2-1 lys2::his3-Δ4 trp1-901 ade1 cac3-1 hir3-2
DC042 MATα ade1 leu2
W303 MAT a ura3-1 leu2-3,112 ade2-1 trp1-1 his3-11,15 can1-100
W303Δ3 MAT a hir3::HIS3 ura3-1 leu2-3,112 ade2-1 trp1-1 his3-11,15 can1-100
UCC4543 MAT a ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 adh4::SUP4-o-TELb
L1698UCC4543hir1::URA3
L1699UCC4543hir2::URA3
L1687UCC4543hir3::LEU2
L1678UCC4543cac3::hisG-URA3-kan-hisG
L1688L1678hir3::LEU2
L1700UCC4543cac3::hisG
L1701L1700hir1::URA3
L1702L1700hir2::URA3
JC364 MAT a ura3-167 his3-Δ200 leu2Δ-hisG trp1Δ-hisGTy588::neo Ty146(tyb::lacZ) Ty1-270his3-AI
L1689JC364cac3::hisG-URA3-kan-hisG
L1690L1689hir3::LEU2
YJB2306 MAT a /MATα ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1
  • a DC042, W303, W303Δ3, UCC4543, and JC364 were kindly provided by M. Olson, R. Rothstein, M. A. Osley, D. Gottschling, and D. Garfinkel, respectively.

  • b SUP4-o-TEL, SUP4-o located near telomere VII-L.