Table 2.

Mutations in hir and cac genes cause a synthetic increase in the rate of Ty1 transposition

Strain/plasmidaRelevant noncomplemented mutation(s)Transposition ratebAvg. increase (fold) in transposition rate
L1356/pCAC3Wild type1.9 (5.0) × 10−8 1.0
L1356/YCp50 cac3-1 4.5 × 10−8 ± 2.4 × 10−8 1.3
L1561/pHIR3 cac3-1 4.0 × 10−8 ± 1.6 × 10−8 1.1
L1675/pHIR3 cac3-1 3.5 (4.3) × 10−8 1.1
L1561/pCAC3 hir3-2 4.8 × 10−8 ± 2.0 × 10−8 1.4
L1675/pCAC3 hir3::LEU2 3.1 (4.9) × 10−8 1.2
L1561/YCp50 cac3-1 hir3-2 6.2 × 10−7 ± 3.0 × 10−7 18.0
L1675/YCp50 cac3-1 hir3::LEU2 3.7 (3.3) × 10−7 10.1
L1685/pCAC3 cac2::TRP1 hir3::LEU2 5.8 (4.5) × 10−7 14.9
L1635/pCAC3 rad6::LEU2 6.0 × 10−8 ± 4.0 × 10−8 1.7
  • a All strains are derivatives of L1356, which was found to contain a relevant mutation, cac3-1. Strains were transformed with the indicated plasmids.

  • b Ty1 transposition rates were determined during growth on −URA in order to maintain selection for the plasmids (see Materials and Methods). When three or more determinations were made, the average and standard deviation are indicated; when two determinations were made, the duplicate value is shown in parentheses.