Table 3.

EMS-induced mutation frequencies in the wild-type andrfc2-1 mutant strains

StrainEMS dose (μg/ml)Survival fraction (%)Frequency of Canr mutants (105)a
YVN12 (wild type)0100.00.48 ± 0.10
10097.22.70 ± 0.27
20099.83.69 ± 0.32
40084.46.56 ± 0.51
80079.68.26 ± 0.62
1,60072.316.34 ± 1.09
YVN13 (rfc2-1)0100.01.28 ± 0.16
10077.63.75 ± 0.35
20064.17.24 ± 0.60
40063.59.29 ± 0.73
80046.414.01 ± 1.16
1,60024.820.83 ± 2.21
  • a Mutation frequencies, expressed as means ± standard deviations, were measured at 25°C.