Table 1.

Effect of glucose or snf1Δ on meiosis

GenotypeaMediumbFrequency of intragenic recombinants (10−5)c% of cells forming sporesd
Wild typeGrowth0.6 ± 0.20
OAc60 ± 1147 ± 2
OAc + Glu0.4 ± 0.10
snf1ΔOAc0.4 ± 0.10
OAc + Glu0.6 ± 0.20
Wild type(YEp-IME1)OAc67 ± 248 ± 3
Glu17 ± 36 ± 3
snf1Δ(YEp-IME1)OAc4.2 ± 0.90
Glu7.8 ± 0.80.2 ± 0.1
  • a The wild type is SH777,snf1Δ issnf1Δ::URA3/snf1Δ::URA3, and wild type (YEp-IME1) and snf1Δ(YEp-IME1) contain the YEp351-IME1 plasmid. Strains are otherwise isogenic.

  • b Growth, YPA medium; OAc, sporulation medium; OAc + Glu, sporulation medium with 0.4% glucose added; Glu, 2% glucose.

  • c Commitment to intragenic recombination between trp1 heteroalleles (Trp+prototrophs/CFU) after 36 h in growth medium or after an additional 24 h in OAc, OAc + Glu, or Glu. Percentages of cell viability for each of the nine cultures were as follows (from top to bottom): 38 ± 2, 37 ± 1, 49 ± 1, 33 ± 1, 38 ± 3, 34 ± 5, 30 ± 6, 33 ± 1, and 19 ± 1.

  • d Percentages of the total population of cells that formed asci, measured at the same times as recombination.