Table 1.

Quantified two-hybrid interactions between Eed and Enx1a

Constructβ-gal activity (MU)b in:
Expt 1Expt 2
Eed5′GAL4DBD + Enx1/1.134.2 (0.98)37.5 (0.15)
Eedhypomorph5′GAL 4DBD + Enx1/1.121 (0.23)20.0 (0.33)
Eedhypomorph5′GAL 4DBD + pPC860 0.1 (0.04)
  • a Quantitative liquid β-galactosidase assays were performed in triplicate, on log-phase yeast cells containing the indicated two-hybrid plasmids.

  • b Data are presented in Miller units (MU) as explained in Materials and Methods, with standard deviations in parentheses.