Table 1.

β-Galactosidase activities of Enx1 and EED interactions in the two-hybrid system

GAL4-DBD fusion (aa)GAL4-TAD fusion (aa)Colony coloraRelative β-galacto- sidase activityb
Enx1 (1–746)EED (1–535)Blue100
HPC2 (1–558)White<1
BMI1 (1–326)White<1
Xbm1 (1–326)White<1
RING1 (1–377)White<1
HPH1 (1–676)White<1
HPH1 (713–1013)White<1
HPH2 (137–432)White<1
EED (1–535)Enx1 (1–746)Blue100
HPC2 (1–558)White<1
BMI1 (1–326)White<1
Xbm1 (1–326)White<1
RING1 (1–377)White<1
HPH1 (1–676)White<1
HPH1 (713–1013)White<1
HPH2 (137–432)White<1
  • a White colonies were obtained on medium lacking both histidine and 3-AT. Blue colonies were obtained on medium lacking histidine but in the presence of 3-AT.

  • b The average β-galactosidase activity in a triplicate experiment was 135 U. This activity was set at 100%.