Table 1.

HEF1 associates specifically with the mitotic regulatory protein hsDim1pa

LexA fusionValue for construct
hsDim1p-activation domainActivation domain vector
Specificity of interaction
 HEF1102–229 572 ± 32421 ± 9
 p85ΔSH25 ± 24 ± 1
 SHC12 ± 39 ± 3
 IRS13 ± 14 ± 4
 Insulin receptor3 ± 14 ± 1
 Bicoid7 ± 46 ± 3
Mapping of site on HEF1
 HEF11–105 5 ± 16 ± 1
 HEF11–124 153 ± 328 ± 1
 HEF11–154 109 ± 2213 ± 2
 HEF1102–175 20 ± 45 ± 2
 HEF1102–229 640 ± 4529 ± 2
 HEF1125–229 50 ± 518 ± 2
 HEF1151–229 45 ± 822 ± 4
 Bicoid4 ± 14 ± 0
 Alone4 ± 16 ± 0
  • a EGY48 yeast cells were transformed with the pJK103 LexA operator-LacZ reporter and indicated combinations of LexA-fused and activation-domain-fused (pJG4-5) proteins. β-Galactosidase activities were calculated as the average values for six separate colonies for each data point. Numbers shown are the β-galactosidase values (± standard deviations) obtained for different constructs.