Table 2.

Two-hybrid interaction of MOB1 with DBF2 and CAF1a

LexA fusionB42 fusionβ-gal (U/mg)b
LexA-DBF2(1–561)B42-MOB1(9–314)260
LexA-DBF2(1–561)B42-MOB1(79–314)530
LexA-DBF2(1–561)B42-MOB1(145–314)66
LexA-DBF2(1–561)B42<1
LexAB42-MOB1(9–314)<1
LexA-DBF2(1–220)B42-MOB1(9–314)59
LexA-DBF2(1–220)B42-MOB1(145–314)120
LexA-DBF2(1–220)B42<1
LexA-MOB1(9–314)B42-DBF2(1–561)6,700
LexA-MOB1(9–314)B42-DBF2(205–561)350
LexA-MOB1(9–314)B42-CAF1300
LexA-MOB1(9–314)B4281
  • a Strains were grown on minimal medium lacking uracil, histidine, and tryptophan and supplemented with 2% raffinose and 2% galactose as previously described (8). All assays were done in EGY188/EGY191 diploids containing the p34 reporter (eight LexA operators upstream of the GAL1-lacZ promoter). B42-DBF2(1–561) and B42-DBF2(205–561) were expressed to comparable levels as determined by Western analysis (data not shown).

  • b β-Galactosidase (β-Gal) activities represent the average of determinations for at least three separate transformants. The standard error of the mean was less than 20% in each case.