Table 1.

Yeast strains

StrainGenotypeSource or reference
EGY48 MATα his3 leu2::3LexAop-LEU2 ura3 trp1 LYS2 13
W3031b MATα ade 2-1 can1-100 leu2-3,112 his3-11,15 trp1-1 ura3-52 P. Walter, University of California
AWY500Same as W3031b excepthis3-11,15::HIS+ UPRE-lacZ This study
AWY446Same as W3031b excepthis3-11,15::HIS+ UPRE-lacZ ptc2-1::kan r This study
AWY506Same as W3031b excepthis3-11,15::HIS+ UPRE-lacZ ptc2-Δ1::LEU2 This study
AWY516Same as W3031b except his3-11,15::HIS+ UPRE-lacZ ire1-Δ1 This study