Table 2.

Mating and sporulation of mei4-defective andmei4-overexpressing strains

ExptaStrainRelevant genotypeZygotes (%)Asci (%)bNuclear type of zygotes (%)c
4 spore2 spore1N(r)1N(ht)2N3-4N
1JZ878 h90mei4 + 1060045190
YW917 h90mei4Δ 6500762400
YW917 h90 mei4Δ [pREP(mei4 +)]13478135280
2YW917 h90 mei4Δ [pAL19]6900287110
YW917 h90 mei4Δ [pAL(mei4 +)]2529516322032
YW917 h90 mei4Δ [pAL(mei4-K85Q)]5600386110
YW917 h90 mei4Δ [pAL(mei4-W115S)]6300406000
YW917 h90 mei4Δ [pAL(mei4-F125D)]5500376210
  • a In experiment 1 cells were cultured on SSA medium for 2 days; in experiment 2 cells were incubated in PM liquid medium lacking N for 12 h.

  • b Two- and four-spore asci were differentially counted.

  • c 1N(r), mononucleate zygotes containing a round nucleus; 1N(ht), mononucleate zygotes with a horsetail nucleus; 2N, binucleate zygotes; 3-4N, tri- or tetranucleate zygotes.