Table 1.

Transgene expression and onset of tumors in MMTV/MT-Y250F micea

LineExpression of transgeneb in:Avg onset of mammary tumors (days)cTumor types and hyperplasias
M.Gl.T (female)M.GL.NSal.Epidid.Sem. Ves.Testes
FemaleMale
250-1NANoneNone
250-2++++++ND+++NDNDND136 (26)M.Gl. adenocarcinomas, male reproductive abnormalities (vas deferens?), hypersensitivity to parasitic infections
250-3NoneNone
250-4NoneNone
250-5(a)+++++++++++142 (74)M.Gl. adenocarcinomas
250-5(b)+++NDNDNDNDNDND211 (23)M.Gl. adenocarcinomas
250-6NANoneNone
250-7NANoneNone
250-8NA+NoneNone
  • a RNase protection analysis was performed on 20 μg of total RNA isolated from a variety of organs in the MMTV/MT-Y250F strains as described in Materials and Methods.

  • b Relative levels of transgene expression (as determined by quantitative phosphorimager analysis): −, not detected; +, low; ++, intermediate; +++, high. M.Gl.N, normal mammary gland; M.Gl.T, mammary gland tumor; Sal., salivary gland; Epidid., epididymus; Sem. Ves.; seminal vesicles; NA, not applicable; ND, not determined.

  • c The number of animals analyzed is given in parentheses.