Table 1.

Yeast strains used in this study

StrainGenotypeReference or comments
D150 a ura3-52 leu2-3,112 ade1-100 his4-519 GAL+ BWG1-7A, L. Guarente
YDL302As D150 with URA3 GAL10::dim1 26
BMA38 aade2-1/ade2-1 can1-100/can1-100 his3Δ200/his3Δ200 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 B. Dujon
YDL303As BM38 withdim1Δ::HIS3/DIM1 Full DIM1 deletion made by PCR in strain BMA38
YDL304A a ade2-1 can1-100 his3Δ200 leu2-3,112 trp1-1 ura3-1 dim1Δ::HIS3 + pDL31.42 (URA3 2μmDIM1)Starting strain, segregant from YDL303 containing aURA3 2μm DIM1 plasmid
YDL304Bαade2-1 can1-100 his3Δ200 leu2-3,112 trp1-1 ura3-1 dim1Δ::HIS3 + pDL31.42 (URA3 2μmDIM1)YDL304A sister strain
YDL209 a ade2-1 can1-100 his3Δ200 leu2-3,112 trp1-1 ura3-1 dim1Δ::HIS3 + pTL6 (LEU2 ARS/CEN DIM1)YDL304A in which plasmid pDL31.42 has been shuffled with plasmid pTL6
MBS a ade2 his3 leu2 trp1 ura3 can1 L-0 M-0P. Sarnow (17)
YDL321As MBS with dim1-2 dim1-2 integrated as adim1-2/HIS3 cassette isolated from pTL39
YDL324As MBS with dim1-1 dim1-1 integrated as adim1-1/HIS3 cassette isolated from pTL43
CH1462αade2 ade3 his3 leu2 ura3 can1 21
YDL314As CH1462 with dim1-9 dim1-9integrated as a dim1-9/HIS3 cassette isolated from pTL42
YDL315As CH1462 with dim1-1 dim1-1integrated as a dim1-1/HIS3 cassette isolated from pTL43
YDL300 aura3-52/ura3-52 lys2-801a m b e r/lys2-801a m b e rade2-101o c h r e/ade2-101o c h r e trp1Δ1/trp1Δ1 his3Δ200/his3Δ200 leu2Δ1/leu2Δ1 dim1Δ::URA3/DIM1 23
YDL100A a; as YDL300 + pTL6 (LEU2 ARS/CEN DIM1) dim1Δ::URA3 segregant from YDL300
YDL101A a; as YDL300 + pTL18 (LEU2 ARS/CEN3× Myc-Dim1p) dim1Δ::URA3 segregant from YDL300
YDL102A a; as YDL300 + pTL25 (LEU2 ARS/CEN Dim1p-3× Myc) dim1Δ::URA3segregant from YDL300