Table 1.

Yeast strains used in this study

Lab nameGenotypeSource
YA102 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 trp1-Δ1 gal3 M. Carlson
YA148 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 leu2-Δ1 trp1-Δ63 Δcup1::ura3 C. Guthrie
YB109 MATα ura3-52 his3-Δ200 ade2-101 trp1-Δ1 gal3 leu2-3,112 GAL1::his3-Δ5′ trp1::his3-Δ3′::HOcs lys2 (leaky)This laboratory
YB110YB109 × YA102This laboratory
YB119 MATα ura3-52 his3-Δ200 ade2-101 lys2-801 trp1-289 leu2-3,112 GAL1::his3-Δ5′ This laboratory
YB130 MATα ura3-52 his3-Δ200 ade2-101 trp1-Δ1 gal3 leu2-3,112 GAL1::his3-Δ5′ trp1::his3-Δ3′::HOcs lys2 (leaky)rad9::URA3 rad9 disruption in YB109
YB131 MATα ura3-52 his3-Δ200 ade2-101 trp1-Δ1 gal3 leu2-3,112 GAL1::his3-Δ5′ trp1::his3-Δ3′::HOcs lys2 (leaky) rad9::LEU2 rad9 disruption in YB109
YB132 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 trp1-Δ1 rad9::URA3 rad9 disruption in YA102
YB133 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 leu2-Δ1 trp1-Δ63 Δcup1::ura3 rad9::LEU2 rad9 disruption in YA148
YB134YB130 × YB132This laboratory
YB135YB131 × YB133This laboratory
YB136 MATα ura3-52 his3-Δ200 ade2-101 trp1-Δ1 gal3 leu2-3,112 GAL1::his3-Δ5′ trp1::his3-Δ3′::HOcs lys2 (leaky)rad1::URA3 rad1 disruption in YB109
YB137 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 trp1-Δ1 rad1::URA3 rad1 disruption in YA102
YB138YB136 × YB137This laboratory
YB139 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 leu2-Δ1 trp1-Δ63 Δcup1::ura3 rad9::LEU2 rad9 and rad1 disruptions in YA148
YB140 MATα ura3-52 his3-Δ200 ade2-101 trp1-Δ1 gal3 leu2-3,112 GAL1::his3-Δ5′ trp1::his3-Δ3′::HOcs lys2 (leaky) rad1::URA3 rad9::LEU2 rad9 and rad1 disruptions in YB109
YB141YB139 × YB140This laboratory
YB142 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 leu2-Δ1 trp1-Δ63 Δcup1::ura3 rad9::URA3 rad9 disruption in YA148
YB143 MATα ura3-52 his3-Δ200 ade2-101 trp1-Δ1 gal3 leu2-3,112 GAL1::his3-Δ5′ trp1::his3-Δ3′::HOcs lys2 (leaky)rad9::URA3 rad52-7::LEU2 rad9and rad52 disruptions in YB109
YB144 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 leu2-Δ1 trp1-Δ63 Δcup1::ura3 rad9::URA3 rad52-7::LEU2 rad9 and rad52disruptions in YA148
YB145YB143 × YB144This laboratory
YB146 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 trp1-Δ1 gal3trp1::[his3-Δ3′::HOcs his3-Δ5′]SCE assay in YA102
YB147 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 trp1-Δ1 gal3trp1::[his3-Δ3′::HOcs his3-Δ5′] rad9::URA3 rad9disruption in YB146
YB148YB109 × YA148This laboratory
YB149 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 leu2-Δ1 trp1-Δ63 Δcup1::ura3 rad1::URA3 rad1 disruption in YA148
YB150YB136 × YB149This laboratory
YB151YB130 × YA102This laboratory
YB152 MAT a ura3-52 his3-Δ200 ade2-101 lys2-801 leu2-Δ1 trp1-Δ63 Δcup1::ura3 rad52-7::LEU2 rad52disruption in YA148
YB153 MATα ura3-52 his3-Δ200 ade2-101 trp1-Δ1 gal3 leu2-3,112 GAL1::his3-Δ5′ trp1::his3-Δ3′::HOcs lys2 (leaky)rad52-7::LEU2 rad52 disruption in YB109
YB154YB152 × YB153This laboratory