Table 1.

Strains of S. cerevisiae used in this study

StrainaGenotypebSource
FY86 MATα ura3-52 leu2-Δ1 his3-Δ200 F. Winston
FY250 MATα ura3-52 leu2-Δ1 his3-Δ200 trp1-Δ63 F. Winston
FY251 MAT a ura3-52 leu2-Δ1 his3-Δ200 trp1-Δ63 F. Winston
MCY3639 MATα ura3-52 leu2-3,112 his3-Δ200 lys2-801 mig1Δ2::LEU2 This laboratory
MCY3644Identical to MCY3647, exceptsrb11Δ::LEU2 This laboratory
MCY3647 MATα ura3-52 leu2-3,112 his3-Δ200 lys2-801 This laboratory
MCY3655Identical to FY250, exceptsrb11Δ::LEU2 This work
MCY3657 MATα ura3-52 leu2-Δ1 his3-Δ200 trp1-Δ63 srb10Δ::HIS3 This work
MCY3658 MAT a ura3-52 leu2-Δ1 his3-Δ200 ctk1ΔE::URA3 This work
MCY3659 MAT a ura3-52 leu2-3,112or -Δ1 his3-Δ200 lys2-801 This work
MCY3660 MATα ura3-52 leu2-3,112 or -Δ1 his3-Δ200 mig1Δ2::LEU2 ctk1ΔE::URA3 This work
MCY3661 MAT a ura3-52 leu2-Δ1 his3-Δ200 trp1-Δ63 This work
MCY3662 MAT a ura3-52 leu2-Δ1 his3-Δ200 trp1-Δ63 srb10Δ::HIS3 This work
MCY3663 MATα ura3-52 leu2-Δ1 his3-Δ200 trp1-Δ63 ctk1ΔE::URA3 This work
MCY3664Identical to MCY3663, except ctk1ΔE::ura3::LEU2 This work
MCY3667 MATα ura3-52 leu2-Δ1 his3-Δ200 srb10Δ::HIS3 ctk1ΔE::URA3 This work
MCY3668Identical to MCY3912, exceptsrb10Δ::HIS3 This work
MCY3694Identical to FY250, exceptsrb10-D290A This work
MCY3912 MAT a ura3-52 leu2-3,112 his3-Δ200 lys2-801 trp1-Δ1 ade2-101 This laboratory
CTY10-5d MAT a ade2-101 leu2-Δ1 his3-Δ200 trp1-Δ901 gal4 gal80 URA3::lexAop-lacZ R. Sternglanz
  • a All strains except CTY10-5d are derived from S288C.

  • b srb10Δ::HIS3 andsrb11Δ::LEU2 are the same asssn3Δ1::HIS3 andssn8Δ1::LEU2, respectively (25).