Table 6.

Stimulation of translocations and SCE by HO-induced DSBs

StrainaNo. of exptsAvg viability after HO induction ± SD (%)bAvg frequency of His+ recombinants ± SDAvg no. of stimulated His+ recombinants/106cellsf ± SD
Before HO induction, per Trp+ CFU from YPLcAfter HO induction
Per Trp+ CFU from YPLdPer Trp+ CFU from YPGale
To monitor translocations
RAD9+ diploid (YB110)889 ± 9(4.5 ± 2.3) × 10−6 (1.6 ± 0.2) × 10−4 (2.2 ± 0.3) × 10−4 201 ± 19
rad9 mutant (YB134)375 ± 7(3.8 ± 0.7) × 10−6 (1.8 ± 0.1) × 10−4 (2.3 ± 0.2) × 10−4 173 ± 6
To monitor SCE
RAD9+ (YB146)591 ± 13(7.5 ± 6.0) × 10−5 (7.9 ± 2.0) × 10−4 (8.6 ± 2.3) × 10−4 715 ± 163
rad9::URA3(YB147)477 ± 9(3.8 ± 1.1) × 10−5 (7.1 ± 1.8) × 10−5 (9.3 ± 2.2) × 10−5 35 ± 23
  • a For complete genotypes, see Table 1.

  • b (Trp+ CFU after HO induction/Trp+ CFU before HO induction) × 100.

  • c Number of His+ recombinants before HO induction/number of Trp+ cells at the time of HO induction.

  • d Number of His+ recombinants after HO induction/number of Trp+ cells at the time of HO induction.

  • e Number of His+ recombinants after HO induction/viable Trp+ cell after HO induction.

  • f Average (frequency of His+recombinants after HO induction − frequency of His+recombinants before HO induction) × 106.