Table 2.

Effect of fus3 mutations on Ty1 transposition

StrainRelevant genotypeMarked Ty elementTransposition rateFold effect of fus3 mu- tation
JC297WTc Ty1his3AI-270(3.1 ± 1.4) × 10−7a
JC953 rtt100-1(fus3-187)Ty1his3AI-270(1.2 ± 0.5) × 10−5a 39
JC242WTTy1his3AI-242(2.1 ± 0.8) × 10−7a
JC1516 fus3Δ::hisG Ty1his3AI-242(3.7 ± 1.4) × 10−6a 18
JC515WTTy1ade2AI-515(3.2 ± 1.2) × 10−8b
JC1038 fus3Δ::HIS3 Ty1ade2AI-515(1.8 ± 0.6) × 10−6b 56
JC1065 rad52 Ty1his3AI-270(4.8 ± 1.7) × 10−6a
JC980 fus3-187 rad52 Ty1his3AI-270(1.7 ± 0.6) × 10−5a 4
JC560WTTy2his3AI-1(7.1 ± 4.2) × 10−8a
JC1515 fus3Δ::hisG Ty2his3AI-1(3.7 ± 2.6) × 10−7a 5
JC563WTTy2his3AI-19(3.3 ± 1.6) × 10−8a
JC1510 fus3Δ::hisG Ty2his3AI-19(9.2 ± 6.0) × 10−8a 3
  • a The rate of His+ prototroph formation per cell per generation.

  • b The rate of Ade+ prototroph formation per cell per generation.

  • c WT, wild type.